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Browsing Faculty of Science and Agriculture by Author "Ncube, I."

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Adaptation of xylose fermenting yeasts, isolated from various sources in the Limpopo Province, to improve ethanol production in the biofuel industry

Tshivhase, Munangiwa (University of Limpopo, 2017)

The recent oil crisis and environmental concerns over fossil fuels has led to the development of biofuels from lignocellulosic materials. Two main sugars from lignocellulose that can be used for bioethanol production are ...
Assessment of the effects of environmental contaminants on feral fish populations in the Olifants river system

Lebepe, Jeffrey (University of Limpopo, 2018)

Freshwater ecosystems are the most threatened systems globally, suffering from channel modification, over extraction of water and, of particular concern, pollution. In South Africa, Olifants River is categorised as the ...
Design of smart hydrogels for use as support matrices for immobilisation of cellulases in saccharification of lignocellulose

Mahlale, Vutlhari Lovemore (2016)

Smart hydrogels could facilitate immobilisation of cellulases to allow recovery and decrease enzyme cost in the biofuel industry, as they have a soluble-gel transition. The aim of the study was to design and evaluate ...
Development of a fungal cellulolytic enzyme combination for use in bioethanol production using hyparrhenia spp as a source of fermentable sugars

Ncube, Thembekile (University of Limpopo (Turfloop Campus), 2013)

The current study investigated four fungal species namely Aspergillus niger FGSC A733, Aspergillus versicolor EF23, Penicillium citrinum AZ01 and Trichoderma harzianum NCGR 0509 for their abilities to produce cellulases ...
Development of chromatographic bioseparations based on lectins and supermacroporous affinity cryogels

Raletjena, Moloko Ivonne (2012)

Various cytomorphologic and biochemical markers of apoptosis are found in different compartments (plasma membrane, cytoplasm, nucleus, and mitochondria) of target cells. Although the plasma membrane is an easily accessible ...
Establishing a microbial co-culture for production of cellulase using banana (musa paradisiaca) pseudostem

Mulaudzi, Mulanga Luscious (2020)

In nature, saccharification is done by a variety of microorganisms, secreting a variety of cellulase in addition to other proteins. Co-culturing enables the production of more efficient enzyme preparations that would mimic ...
Evaluation of high recombinant protein secretion phenotype of saccharomyces cerevisiae segregant

Sibanda, Ntsako (University of Limpopo, 2016)

The ever increasing cost of fossil-based fuels and the accompanying concerns about their impact on the environment is driving research towards clean and renewable sources of energy. Bioethanol has the potential to be a ...
Evaluation of recombinant yeast strains expressing a xylanase, amylase or an endo-glucanase in brewing

Makuru, Moshabane Phillip (2018)

Beer is one of the most widely consumed alcoholic beverages in the world. The brewing process is based on natural enzymatic activities that take place during the malting of barley grain, mashing of grist and fermentation ...
Genetic characterisation of colophospermum mopane (sensu lato) using RAPD analyses

Legodi, Mankone Priscilla (University of Limpopo (Turfloop Campus), 2007)

Colophospermum mopane (sensu lato) is currently recognised on morphological and physiological characteristics. To add to the suite of taxonomic characters, the genetic variability of C. mopane (sensu lato) was investigated ...
Heterologous expression of a Mukwa (pterocarpus angolensis ) seed lectin (Pal) gene in Escherichia coli, Saccharomyces cerevisiae and Yarrowia lipolytica and construction of Pal recombinant vector for expression in Aspergillus niger

Ngoepe, Mafora Gloria (University of Limpopo (Turfloop Campus), 2011)

Pterocarpus angolensis seed lectin (PAL), a 28 kDa non glycosylated protein, was initially successfully cloned and expressed in E. coli for ease of high protein production. It was discovered, however, as in similar studies ...
The improvement of bioethanol production by pentose fermenting yeasts previously isolated from herbal preparations, dung beetles and marula wine

Moremi, Mahlatse Ellias (2020)

Production of bioethanol from lignocellulosic biomass has gained significant attention worldwide as an alternative fuel source for the transportation sector without affecting food supply. Efficient conversion of pentose ...
An investigation on the cause of recalcitrance to genetic transformation in soybean,glycline max (L.) merrill

Mangena, Phetolo (University of Limpopo, 2019)

Genetic transformation offers great opportunities for rapidly introducing, selecting or inducing desired characteristics in various leguminous plants for breeding purposes. But, this technique remains aloof for soybean ...
Isolation and characterisation of a galactose-specific lectin from maturing seeds of lonchocarpus capassa and molecular cloning of the lectin gene

Masingi, Nkateko Nhlalala (2010)

A 29 kDa lectin that shows specificity for galactose was isolated from Lonchocarpus capassa seeds by a combination of ammonium sulphate precipitation and affinity chromatography on a galactose-sepharose column. The 29 kDa ...
Morphological and genetic diversity analysis in selected Vernonia lines

Ramalema, Seganka Piet (2007)

Vernonia (Vernonia galamensis) is a new potential industrial oilseed crop. The seeds of this crop contain unusual naturally epoxidised fatty acids which are used in the production of various industrial products. The ...
Morphological and genetic diversity analysis in selected vernonia lines

Ramalema, Seganka Piet (2007)

Vernonia (Vernonia galamensis) is a new potential industrial oilseed crop. The seeds of this crop contain unusual naturally epoxidised fatty acids which are used in the production of various industrial products. The objective ...
Production and characteristics of a b-glucosidase from a thermophilic bacterium and investigation of its potential as part of a cellulase cocktail for conversion of lignocellulosic biomass to fermentable sugars

Masingi, Nkateko Nhlalala (2020)

The use of lignocellulosic biomass for bioethanol production is largely dependent on cost effective production of cellulase enzymes and most importantly, the availability of cellulases with sufficient β-glucosidase ...
Production, characterization and evaluation of fungal cellulases for effective digestion of cellulose

Mokatse, Khomotso (University of Limpopo (Turfloop Campus), 2013)

The production of cellulase is a key factor in the hydrolysis of cellulosic materials and it is essential to make the process economically viable. Cellulases are the most studied multi- enzyme complex and comprise of ...
A proteomic approach for discovery of microbial cellulolytic enzymes

Matlala, Maphuti Sanna (2020)

Bioethanol production from lignocellulosic biomass is seen as an alternative source of energy. However, large-scale production of bioethanol from lignocellulosic biomass is still not feasible due to the high cost of ...
Proteomic approaches to profiling of cysteine proteases expressed in leaves and root nodules during natural senescence of the soybean plant

Karumazondo, Rumbidzai Patience (2011)

Soybean is one of the most cultivated legume plants in developing countries. Nodule senescence is a major limitation in producing high yields of soybean as it coincides with the pod filling stage. Delaying nodule senescence ...
Screening, isolation and characterization of lectins extracted from mushrooms indegenous to Southern Africa

Maribeng, Reagile (2011)

Lectins are among a large number of proteins produced by mushrooms. Mushroom lectins with important biological functions have been isolated and studied. However, none of the studies were reported on lectins isolated from ...

Browsing Faculty of Science and Agriculture by Author "Ncube, I."

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