Show simple item record Mabusa, Itumeleng H. Rachmond, Howard Masoko, Peter 2019-09-12T08:19:46Z 2019-09-12T08:19:46Z 2017
dc.identifier.issn 0254-3486
dc.description Journal article published in the South African Journal for Science and Technology / Suid-Afrikaanse Tydskrif vir Natuurwetenskap en Tegnologie en_US
dc.description.abstract Sutherlandia frutescens (L) R. Br. contains several essential, bioactive compounds with clinically proven pharmacological activities. Sutherlandia is prescribed for people with tuberculosis but it is still not known what compounds in this plant act against Mycobacterium tuberculosis and its mode of action. This study is aimed at determining if S. frutescens extracts contain antimycobacterial compounds. Aerial parts of S. frutescens were dried, ground and extracted with ethanol, dichloromethane: methanol 1:1 (v/v) and water. The chemical profiling was done using highperformance liquid chromatography-mass spectroscopy (HPLC-MS) and thin layer chromatography (TLC). TLC plates were developed in butanol:acetic acid:water (BAW) to the ratio of 21:6:3; chloroform:methanol:water:formic acid (CMWF1) [60:15:2:1] and (CMWF2) [21:9:1:0.3]. Qualitative antioxidant activity was done, using 2.2-diphenylpacryl-1-hydrazyl (DPPH). Antimycobacterial activity of the plant extracts was evaluated, using micro-dilution and bioautographic methods against Mycobacterium smegmatis. Low antimycobacterial activity against M. smegmatis was observed on the bioautograms. The ethanol extracts contained more compounds compared to water extracts on HPLC-MS chromatographic profiles. The average Minimum Inhibitory Concentration (MIC) values for all the extracts were 0.61 mg/mL units and the DCM:MeOH (1:1) extract had the lowest MIC value of 0.28 mg/mL. The results showed that the plant could be further explored for possible antimycobacterial agents. Low activity was observed, possibly due to low replication of bacilli and non-replicating organisms. The study provides preliminary scientific validation of the traditional medicinal use of this plant. Further studies are required to identify the bioactive compounds in the DCM:MeOH 1:1 extract which showed significant antimycobacterial activities. en_US
dc.format.extent 8 pages en_US
dc.language.iso en en_US
dc.publisher South African Journal for Science and Technology / Suid-Afrikaanse Tydskrif vir Natuurwetenskap en Tegnologie en_US
dc.relation.requires Adobe Acrobat Reader en_US
dc.subject Mycobacterium smegmatis en_US
dc.subject Sutherlandia frutescens en_US
dc.subject.lcsh Legumes en_US
dc.subject.lcsh Mycobacterium en_US
dc.subject.lcsh Mycobacterium tuberculosis en_US
dc.title Sutherlandia frutescens (Fabaceae) extracts used for treating tuberculosis do not have high activity against Mycobacterium smegmatis en_US
dc.type Article en_US

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