Show simple item record Singh-Moodley, Ashika Duse, Adriano Naicker, Preneshni Kularatne, Ranmini Nana, Trusha Lekalakala, Ruth Mbelle, Nontombi Dawood, Halima Han, Khine Swe Swe Ramjathan, Praksha Bhola, Prathna Whitelaw, Andrew Perovic, Olga 2020-07-14T09:34:42Z 2020-07-14T09:34:42Z 2018
dc.identifier.issn 1972-2680
dc.description Article published in the Journal of Infection in Developing Countries; vol 12(8):616-624. doi:10.3855/jidc.9539 en_US
dc.description.abstract Introduction: Antimicrobial resistant bacterial infections are widespread globally and increases in antimicrobial resistance presents a major threat to public health. Pseudomonas aeruginosa is an opportunistic healthcare-associated pathogen with high rates of morbidity and mortality and an extensive range of resistance mechanisms. This study describes the antibiotic susceptibility profiles of P. aeruginosa isolates from patients with bacteraemia submitted by sentinel laboratories in South Africa from 2014 to 2015. Methodology: Organism identification and antimicrobial susceptibility testing were done using automated systems. Molecular methods were used to detect common resistance genes and mechanisms. Results: Overall the susceptibility was high for all antibiotics tested with a decrease over the two-year period. There was no change in the MIC50 and MIC90 breakpoints for all antibiotics from 2014 to 2015. The MIC50 was within the susceptible breakpoint range for most antibiotics and the MIC90 was within the susceptible breakpoint range for colistin only. Phenotypically carbapenem non-susceptible isolates harboured the following plasmid-mediated genes: blaVIM (n = 81, 12%) and blaGES (n = 6, 0.9%); blaNDM (n = 4, 0.6%) and blaOXA-48 and variants (n = 3, 0.45%). Porin deletions were observed in one meropenem non-susceptible isolate only, and multi-drug resistance efflux pumps were expressed in the majority of the non-susceptible isolates investigated. BlaVEB-1, blaIMP and blaKPC were not detected. Conclusion: The prevalence of resistance to commonly used antibacterial agents was low for P. aeruginosa isolates and similarly, tested resistance mechanisms were detected in a relatively small proportion of isolates. Findings in this study represent baseline information for understanding antimicrobial susceptibility patterns in P. aeruginosa isolates from blood. Our surveillance report may assist in contributing to hospital treatment guidelines. en_US
dc.format.extent 09 pages en_US
dc.language.iso en en_US
dc.publisher Journal of Infection in Developing Countries en_US
dc.relation.requires pdf en_US
dc.subject Antimicrobial susceptibility testing en_US
dc.subject Resistance genes en_US
dc.subject Carbapenemases en_US
dc.subject Efflux pumps en_US
dc.subject Porins en_US
dc.subject.lcsh Medical-Microbiology en_US
dc.subject.lcsh Pathogenic micro-organisms en_US
dc.title Laboratory based antimicrobial resistance surveillance for Pseudomonas aeruginosa blood isolates from South Africa en_US
dc.type Article en_US

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