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dc.contributor.advisor Opuwari, C. S. Takalani, Ndivhuho Beauty
dc.contributor.other Adefolaju, G. A.
dc.contributor.other Henkel, R. 2022-06-27T12:30:53Z 2022-06-27T12:30:53Z 2020
dc.description Thesis (M. Sc. (Medical Sciences)) -- University of Limpopo, 2020 en_US
dc.description.abstract This study investigated the effect of rooibos tea on human sperm in vitro. Semen samples from 50 healthy men and 50 patients were treated with aqueous extracts of fermented rooibos (0 µg/ml, 0.10 µg/ml, 1.0 µg/ml, 10 µg/ml, 100 μg/ml) and unfermented rooibos (0 µg/ml, 0.15 µg/ml, 1.5 µg/ml, 15 µg/ml, 150 μg/ml) for one hour at 37ºC. Thereafter, sperm parameters were analysed. Exposure to unfermented rooibos had no effect on sperm motility, vitality and DNA fragmentation in both groups (P>0.05). However, this treatment significantly increased the production of reactive oxygen species, thus influencing the acrosome reaction, and demonstrated a decrease in mitochondrial membrane potential in the donor group (P<0.05). Treatment with fermented rooibos increased progressive motility in the donor group (P<0.05) but had no effect on sperm vitality, reactive oxygen species and the acrosome reaction in either group (P>0.05). A decrease in mitochondrial membrane potential and DNA fragmentation was observed in the donor group and the patient group (P<0.05). Rooibos tea maintains certain sperm functional parameters, and this may positively affect fertility rate. en_US
dc.description.sponsorship National Research Foundation (NRF) en_US
dc.format.extent xv, 192 leaves en_US
dc.language.iso en en_US
dc.relation.requires PDF en_US
dc.subject Effects of rooibos tea en_US
dc.subject Sperm cells en_US
dc.subject Men en_US
dc.subject.lcsh Antioxidants en_US
dc.subject.lcsh Rooibos tea en_US
dc.subject.lcsh Spermatozoa en_US
dc.title In vitro effects of aqueous extract of aspalathus linearis (rooibos) on human sperm cells en_US
dc.type Thesis en_US

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