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dc.contributor.advisor Mampuru, Leseilane J. Gololo, Sechene Stanley
dc.contributor.other Eloff, Kobus
dc.contributor.other Masoko, Peter
dc.contributor.other Mokgotho, Matlou P. 2012-09-12T09:07:05Z 2012-09-12T09:07:05Z 2008
dc.description Thesis (M.Sc.) --University of Limpopo, 2008 en_US
dc.description.abstract Senna species, a member of the Fabaceae family (subfamily Caesalpinaceae), is widely used traditionally to treat a number of disease conditions such as sexually transmitted diseases and some forms of intestinal complications. In this study the roots of Senna species, collected from Zebediela region of the Limpopo province (R.S.A), were ground to a fine powder and extracted with acetone by cold/shaking extraction method. The phytochemical composition of the extract was then determined by thin layer chromatography (TLC). The chromatograms were visualised with vanillin-sulphuric acid and p-anisaldehyde reagents. The total phenolic content of the extract was determined by Folin-Ciocalteu method and expressed as TAE/g of dry plant material. The extract was assayed for the in vitro anticancer activity using Jurkat T cells. The antioxidant activity was evaluated using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay and the antibacterial activity determined by both bioautographic and the microtiter plate methods. The acetone extract of the roots of Senna species inhibited the growth of Jurkat T cells in a dose- and time-dependent manner. The extract was shown to possess free radical scavenging activity and antibacterial activity against Pseudomonas aeruginosa, Enterococcus faecalis, Escherichia coli and Staphylococcus aureus with MIC values of 0.16, 0.078, 0.078 and 0.16 mg/ml, respectively. A compound with free radical scavenging activity was isolated from the acetone extract of the roots of Senna species through bioassay-guided fractionation. The isolated compound was identified as 1, 3-diphenol-2-propen-1-one. Thus, the study has systematically shown the biological activity of the roots of Senna species and the isolation and identification of the bioactive compound. en_US
dc.format.extent xii, 78 leaves : col. ill. en_US
dc.language.iso en en_US
dc.publisher University of Limpopo en_US
dc.relation.requires PDF en_US
dc.subject Senna species en_US
dc.subject.ddc 583.749 en_US
dc.subject.lcsh Ethnobotany -- South Africa en_US
dc.subject.lcsh Medicinal plants en_US
dc.title Biological activity analysis of the crude extract of the Senna species : structure elucidation of a compound with antioxidant activity en_US
dc.type Thesis en_US

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